Abstract
Renal 25-hydroxyvitamin D3 1alpha-hydroxylase [1alpha(OH)ase] catalyzes metabolic activation of 25-hydroxyvitamin D3 into 1alpha, 25-dihydroxyvitamin D3 [1alpha,25(OH)2D3], an active form of vitamin D, and is inhibited by 1alpha,25(OH)2D3. 1alpha(OH)ase, which was cloned from the kidney of mice lacking the vitamin D receptor (VDR-/- mice), is a member of the P450 family of enzymes (P450VD1alpha). Expression of 1alpha(OH)ase was suppressed by 1alpha, 25(OH)2D3 in VDR+/+ and VDR+/- mice but not in VDR-/- mice. These results indicate that the negative feedback regulation of active vitamin D synthesis is mediated by 1alpha(OH)ase through liganded VDR.
MeSH terms
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25-Hydroxyvitamin D3 1-alpha-Hydroxylase / genetics*
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25-Hydroxyvitamin D3 1-alpha-Hydroxylase / metabolism*
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Amino Acid Sequence
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Animals
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COS Cells
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Calcifediol / metabolism
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Calcitriol / biosynthesis*
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Calcitriol / metabolism
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Calcitriol / pharmacology
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Cloning, Molecular
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Feedback
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Gene Expression Regulation, Enzymologic*
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Kidney / enzymology
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Kidney / metabolism
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Ligands
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Mice
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Mice, Knockout
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Molecular Sequence Data
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptors, Calcitriol / metabolism
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Transfection
Substances
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Ligands
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RNA, Messenger
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Receptors, Calcitriol
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25-Hydroxyvitamin D3 1-alpha-Hydroxylase
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Calcitriol
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Calcifediol
Associated data
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GENBANK/AB005989
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GENBANK/AB006034