Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I

FEMS Microbiol Lett. 1997 Sep 1;154(1):81-8. doi: 10.1111/j.1574-6968.1997.tb12627.x.

Abstract

The Corynebacterium glutamicum glutamine synthetase I (GSI) structural gene glnA was cloned by a PCR approach using oligonucleotide primers derived from conserved amino acid sequences of the GSI proteins from various bacteria. Disruption or deletion of this gene in C. glutamicum led to a glutamine auxotrophic phenotype and complete loss of glutamine synthetase activity, indicating the key role of this enzyme in nitrogen metabolism. Additionally, indications for a second glutamine synthetase, GSII, were found.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / metabolism
  • Blotting, Southern
  • Cloning, Molecular
  • Corynebacterium / enzymology*
  • Corynebacterium / genetics*
  • DNA, Bacterial / analysis
  • Gene Deletion
  • Glutamate-Ammonia Ligase / genetics*
  • Molecular Sequence Data
  • Mutagenesis
  • Nitrogen / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • Amino Acids
  • DNA, Bacterial
  • glutamine synthetase 2
  • glutamine synthetase I
  • Glutamate-Ammonia Ligase
  • Nitrogen

Associated data

  • GENBANK/Q10377
  • GENBANK/Q10378
  • GENBANK/Y13221