Abstract
Class I MHC protein is induced in glia but not mature neurons by IFN-gamma. We have compared IFN-gamma signal transduction in these populations. There were identical levels of STAT1 homodimers and IRF-1 by gel-shift and IRF-1 mRNA was induced equally. However class I MHC, beta2-microglobulin and interleukin 1-beta converting enzyme mRNA levels were greatly reduced in neurons. These experiments show that there is no defect in expression of IRF-1 in response to IFN-gamma in mature mouse neurons but that insufficient class I MHC gene expression is induced for detectable cell surface protein expression.
MeSH terms
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Animals
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DNA-Binding Proteins / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Gene Expression Regulation / drug effects
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Histocompatibility Antigens Class I / metabolism
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Interferon Regulatory Factor-1
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Interferon-gamma / pharmacology*
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Mice / embryology
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Neuroglia / drug effects
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Neuroglia / metabolism*
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Neurons / drug effects
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Neurons / metabolism*
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Phosphoproteins / metabolism*
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Polymerase Chain Reaction
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RNA, Messenger
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Transcription Factors / metabolism
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Transcription Factors / physiology
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Transcription, Genetic
Substances
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DNA-Binding Proteins
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Histocompatibility Antigens Class I
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Interferon Regulatory Factor-1
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Irf1 protein, mouse
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Phosphoproteins
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RNA, Messenger
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Transcription Factors
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Interferon-gamma