Abstract
The value of intratumoral thymidylate synthase (TS) quantitation as a predictive parameter for hepatic artery infusion (HAI) chemotherapy in patients with colorectal liver metastases was investigated. Relative TS mRNA levels were determined in 29 tumor samples using a quantitative RT-PCR amplification method. The median level of expression was 3.0 x 10(-3) (no units) and varied considerably among the tumors over a range of 135-fold. Patients with low TS levels were 4.1-fold more likely to respond (P < 0.03) compared to patients with high TS levels. Our results indicate that TS quantitation is a valuable predictive marker for tumor response to HAI therapy.
Publication types
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Clinical Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Antimetabolites, Antineoplastic / administration & dosage
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Antimetabolites, Antineoplastic / therapeutic use*
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Antineoplastic Combined Chemotherapy Protocols / administration & dosage
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Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
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Biomarkers
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Colorectal Neoplasms / drug therapy*
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Colorectal Neoplasms / enzymology
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Drug Administration Schedule
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Drug Resistance, Neoplasm
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Epirubicin / administration & dosage
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Female
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Floxuridine / administration & dosage
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Floxuridine / therapeutic use*
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Fluorouracil / administration & dosage
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Fluorouracil / therapeutic use*
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Hepatic Artery
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Humans
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Infusions, Intra-Arterial
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Leucovorin / administration & dosage
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Leucovorin / therapeutic use
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Liver Neoplasms / drug therapy*
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Liver Neoplasms / enzymology
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Liver Neoplasms / secondary*
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Male
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Middle Aged
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Mitoxantrone / administration & dosage
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Polymerase Chain Reaction / methods
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Predictive Value of Tests
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Prognosis
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RNA, Messenger / biosynthesis
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Survival Rate
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Thymidylate Synthase / analysis
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Thymidylate Synthase / biosynthesis*
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Transcription, Genetic
Substances
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Antimetabolites, Antineoplastic
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Biomarkers
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RNA, Messenger
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Floxuridine
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Epirubicin
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Mitoxantrone
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Thymidylate Synthase
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Leucovorin
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Fluorouracil