Abstract
Epidemiological data have demonstrated rapid growth of HIV-1 infections among injecting drug users (IDUs) in the Ukraine and Russia, during 1996. Here we describe the results of genetic analysis of isolates derived from 12 HIV-1-infected IDUs in different sites of Russia and the Ukraine. The blood samples were taken within a 1- to 2-month period after the first HIV-1-positive test. The results of the heteroduplex mobility assay as well as gag/env phylogenetic analysis reveal that all sequences belong to gag/env genetic subtype A. Moreover, interpatient genetic distances between the nucleotide sequences encompassing the C2-V3, the V4-V5, and p17-encoding regions within this group were low (the average means were 0.9, 1.3, and 0.4%, respectively). These data show a marked homogeneity of HIV-1, probably spreading during primary infection. It is possible that the current epidemic of subtype A HIV-1 among IDUs in the former Soviet Union is caused by a point source exposure.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Amino Acid Sequence
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Disease Outbreaks*
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Female
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Gene Products, env / analysis
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Gene Products, env / chemistry
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Gene Products, env / genetics
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Gene Products, gag / analysis
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Gene Products, gag / chemistry
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Gene Products, gag / genetics
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HIV Infections / blood
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HIV Infections / epidemiology*
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HIV Infections / genetics
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HIV-1 / classification
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HIV-1 / genetics*
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HIV-1 / isolation & purification
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Humans
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Male
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Molecular Sequence Data
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Phylogeny
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Russia / epidemiology
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Sequence Homology, Amino Acid
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Substance Abuse, Intravenous / physiopathology
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Substance Abuse, Intravenous / virology*
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Ukraine / epidemiology
Substances
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Gene Products, env
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Gene Products, gag
Associated data
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GENBANK/U93611
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GENBANK/U93612
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GENBANK/U93613
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GENBANK/U93614
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GENBANK/U93615
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GENBANK/U93616
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GENBANK/U93617
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GENBANK/U93618
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GENBANK/U93619
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GENBANK/U93620
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GENBANK/U93621
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GENBANK/U93622
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GENBANK/U93623
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GENBANK/U93624
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GENBANK/U93625
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GENBANK/U93626
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GENBANK/U93627
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GENBANK/U93628
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GENBANK/U93629
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GENBANK/U93630
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GENBANK/U93631
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GENBANK/U93632
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GENBANK/U93633
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GENBANK/U93634
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GENBANK/U93635
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GENBANK/U93636
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GENBANK/U93637
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GENBANK/U93638
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GENBANK/U93639
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GENBANK/U93640