Assay of vitamin D derivatives and purification of vitamin D hydroxylases

Methods Enzymol. 1997:282:186-99. doi: 10.1016/s0076-6879(97)82107-6.

Authors

Y Ohyama¹, S Hayashi, E Usui, M Noshiro, K Okuda

Affiliation

¹ Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Japan.

PMID: 9330288
DOI: 10.1016/s0076-6879(97)82107-6

No abstract available

MeSH terms

Animals
Cholestanetriol 26-Monooxygenase
Chromatography
Chromatography, High Pressure Liquid / methods
Chromatography, Ion Exchange
Cytochrome P-450 Enzyme System / analysis
Cytochrome P-450 Enzyme System / isolation & purification*
Cytochrome P-450 Enzyme System / metabolism
Durapatite
Female
Indicators and Reagents
Kidney / enzymology*
Kinetics
Microsomes, Liver / enzymology*
Mitochondria / enzymology*
Mitochondria, Liver / enzymology*
Rats
Steroid Hydroxylases / analysis
Steroid Hydroxylases / isolation & purification*
Steroid Hydroxylases / metabolism
Vitamin D3 24-Hydroxylase

Substances

Indicators and Reagents
Cytochrome P-450 Enzyme System
Durapatite
Steroid Hydroxylases
Cholestanetriol 26-Monooxygenase
Vitamin D3 24-Hydroxylase