Molecular cloning of vitamin D3 hydroxylases

Methods Enzymol. 1997:282:213-23. doi: 10.1016/s0076-6879(97)82109-x.

Authors

M Noshiro¹, Y Ohyama, E Usui, M Akiyoshi-Shibata, Y Yabusaki, K Okuda

Affiliation

¹ Department of Biochemistry, University School of Dentistry, Hiroshima University, Japan.

PMID: 9330290
DOI: 10.1016/s0076-6879(97)82109-x

No abstract available

MeSH terms

Alcohol Dehydrogenase / biosynthesis
Animals
Base Sequence
COS Cells
Cholestanetriol 26-Monooxygenase
Cloning, Molecular / methods
Cytochrome P-450 Enzyme System / biosynthesis*
Cytochrome P-450 Enzyme System / isolation & purification
Cytochrome P-450 Enzyme System / metabolism
DNA, Complementary
Escherichia coli
Kinetics
Liver / enzymology*
Molecular Sequence Data
Oligodeoxyribonucleotides
Plasmids
Promoter Regions, Genetic
Rats
Recombinant Fusion Proteins / biosynthesis
Recombinant Fusion Proteins / isolation & purification
Recombinant Fusion Proteins / metabolism
Saccharomyces cerevisiae / enzymology
Steroid Hydroxylases / biosynthesis*
Steroid Hydroxylases / isolation & purification
Steroid Hydroxylases / metabolism
Substrate Specificity
Vitamin D3 24-Hydroxylase

Substances

DNA, Complementary
Oligodeoxyribonucleotides
Recombinant Fusion Proteins
Cytochrome P-450 Enzyme System
Alcohol Dehydrogenase
Steroid Hydroxylases
Cholestanetriol 26-Monooxygenase
Vitamin D3 24-Hydroxylase