In this study, the development of myelination in the fetal bovine brain (age-range: between 1-2 and 8-9 months) was examined applying: 1. Immunohistochemical staining methods and antibodies against bovine proteolipid protein (PLP), synthetic tridecapeptide of bovine PLP, human myelin basic protein (MBP), human myelin-associated glycoprotein (MAG); and 2. Using the Luxol fast-blue (LFB) technique.