Transfer of small YACs to E. coli as large circular plasmids

Genet Anal. 1997 Jul;14(2):55-9. doi: 10.1016/s1050-3862(97)00012-0.

Abstract

We have designed a YAC circularization vector, pCIRC3, allowing enrichment of the YAC DNA by exonuclease digestion of the linear yeast chromosomes. Due to the presence of P1 replicon sequences in this vector, the circular YACs would replicate as PACs in Escherischia coli.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosomes, Artificial, Yeast*
  • DNA, Circular*
  • Escherichia coli / genetics*
  • Plasmids*

Substances

  • DNA, Circular