Construction of an approximately 2 Mb contig in the region around 80 cM of Arabidopsis thaliana chromosome 2

M L Wang; L Huang; D K Bongard-Pierce; S Belmonte; E A Zachgo; J W Morris; M Dolan; H M Goodman

doi:10.1046/j.1365-313x.1997.00711.x

Construction of an approximately 2 Mb contig in the region around 80 cM of Arabidopsis thaliana chromosome 2

Plant J. 1997 Sep;12(3):711-30. doi: 10.1046/j.1365-313x.1997.00711.x.

Authors

M L Wang¹, L Huang, D K Bongard-Pierce, S Belmonte, E A Zachgo, J W Morris, M Dolan, H M Goodman

Affiliation

¹ Department of Genetics, Harvard Medical School, Boston, MA, USA.

PMID: 9351255
DOI: 10.1046/j.1365-313x.1997.00711.x

Abstract

A method for construction of bacterial artificial chromosome (BAC) contigs from a yeast artificial chromosome (YAC) physical map is described. An approximately 2 Mb contig, consisting of two large BAC contigs linked by a small YAC, has been assembled in the region around 80 cM of Arabidopsis thaliana chromosome 2. Clones from this contig will facilitate gene isolation in the region and can be used directly as substrates for DNA sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / genetics*
Chloroplasts / genetics
Chromosome Mapping
Chromosomes*
Chromosomes, Artificial, Yeast
Cloning, Molecular
DNA, Plant / chemistry*
DNA, Ribosomal / chemistry
Sequence Analysis, DNA

Substances

DNA, Plant
DNA, Ribosomal