Abstract
The anti-proliferative effect of methylglyoxal bis(cyclopentylamidino-hydrazone) (MGBCP), a multi-enzyme inhibitor of polyamine biosynthesis, on the growth of Helicobacter pylori was investigated. MGBCP inhibited the cell growth of H. pylori in a dose-dependent manner. The inhibition was partially reversed by the addition of spermidine. Synthesis of macromolecules, DNA, RNA and protein, was inhibited in the spermidine-depleted H. pylori cells. These findings suggest that MGBCP exhibits an anti-proliferative effect on H. pylori by suppression of macromolecule synthesis.
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / biosynthesis
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DNA, Bacterial / biosynthesis
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Enzyme Inhibitors / pharmacology
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Helicobacter Infections / drug therapy
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Helicobacter Infections / microbiology
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Helicobacter pylori / drug effects*
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Helicobacter pylori / growth & development
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Helicobacter pylori / metabolism*
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Humans
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In Vitro Techniques
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Mitoguazone / analogs & derivatives*
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Mitoguazone / pharmacology
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Polyamines / metabolism*
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Putrescine / biosynthesis
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RNA, Bacterial / biosynthesis
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Spermidine / biosynthesis
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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Enzyme Inhibitors
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Polyamines
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RNA, Bacterial
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methylglyoxal bis(cyclopentylamidinohydrazone)
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Mitoguazone
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Spermidine
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Putrescine