Reduced lipid peroxidation and ischemia-reperfusion injury after lung transplantation using low-potassium dextran solution for lung preservation

Am J Respir Crit Care Med. 1997 Oct;156(4 Pt 1):1073-81. doi: 10.1164/ajrccm.156.4.9607109.

Abstract

Ischemia-reperfusion injury is one of the significant problems in clinical lung transplantation. We investigated the effect of lung preservation with Euro-Collins solution (EC group) or low-potassium dextran solution (LPD group) on lipid peroxidation and ischemia-reperfusion injury in a pig model of lung allotransplantation. The donor lungs were preserved at 4 degrees C for 18 h. Left-sided single lung transplantation was performed, followed by 6 h of reperfusion. Lipid peroxidation was measured as thiobarbituric acid-reactive materials (TBARM) in bronchoalveolar lavage (BAL) fluid and effluent solutions from pulmonary artery (Effluent). After 18 h of ischemia, the LPD group showed lower TBARM in BAL and Effluent than the EC group (p < 0.05). After ischemia plus reperfusion, lung wetto-dry weight ratios and TBARM levels in BAL in the LPD group were lower than those of the EC group (p < 0.05). Lung wet-to-dry weight ratios correlated with TBARM levels in BAL (p < 0.05, r = 0.50). We conclude lipid peroxidation in BAL and Effluent may reflect the degree of ischemia-reperfusion injury, and lung preservation with LPD can reduce lipid peroxidation and lung injury as compared with EC.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bronchoalveolar Lavage Fluid / chemistry
  • Dextrans / pharmacology
  • Glucose / pharmacology
  • Hemodynamics
  • Hypertonic Solutions / pharmacology
  • Leukocyte Count
  • Lipid Peroxidation / drug effects*
  • Lipid Peroxidation / physiology
  • Lung Transplantation / adverse effects*
  • Neutrophils / pathology
  • Organ Preservation / methods*
  • Organ Preservation Solutions / pharmacology*
  • Potassium
  • Reperfusion Injury / etiology
  • Reperfusion Injury / physiopathology
  • Reperfusion Injury / prevention & control*
  • Swine
  • Thiobarbituric Acid Reactive Substances / analysis

Substances

  • Dextrans
  • Euro-Collins' solution
  • Hypertonic Solutions
  • Organ Preservation Solutions
  • Thiobarbituric Acid Reactive Substances
  • low potassium dextran glucose solution
  • Glucose
  • Potassium