Developing lymph nodes collect CD4+CD3- LTbeta+ cells that can differentiate to APC, NK cells, and follicular cells but not T or B cells

Immunity. 1997 Oct;7(4):493-504. doi: 10.1016/s1074-7613(00)80371-4.

Abstract

For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. We show here that CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1 and that can become natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. Since the necessity of lymphotoxin in lymphoid organ development has been shown, we propose that the novel subset of CD4+CD3-LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Antigen-Presenting Cells / cytology*
  • B-Lymphocytes / cytology
  • CD3 Complex / metabolism*
  • CD4-Positive T-Lymphocytes / cytology*
  • Cell Adhesion Molecules
  • Cytotoxicity, Immunologic
  • Fluorescent Antibody Technique, Indirect
  • GTP-Binding Proteins / genetics
  • Gene Expression
  • Histocompatibility Antigens Class II / metabolism
  • Immunity, Cellular
  • Immunoglobulins / metabolism
  • Integrins / metabolism
  • Interleukin-2 / pharmacology
  • Killer Cells, Natural / cytology*
  • Leukocyte Common Antigens / analysis
  • Leukopoiesis*
  • Lymph Nodes / cytology
  • Lymph Nodes / embryology*
  • Lymphocyte Subsets / cytology*
  • Lymphotoxin-alpha / metabolism*
  • Lymphotoxin-beta
  • Membrane Proteins / metabolism*
  • Mice
  • Mice, Inbred AKR
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mucoproteins / metabolism
  • RNA, Messenger / genetics
  • Receptors, CXCR5
  • Receptors, Chemokine
  • Receptors, Cytokine / genetics
  • Spleen / embryology
  • Spleen / immunology
  • T-Lymphocytes / cytology

Substances

  • CD3 Complex
  • CXCR5 protein, mouse
  • Cell Adhesion Molecules
  • Histocompatibility Antigens Class II
  • Immunoglobulins
  • Integrins
  • Interleukin-2
  • LTB protein, human
  • Ltb protein, mouse
  • Lymphotoxin-alpha
  • Lymphotoxin-beta
  • Madcam1 protein, mouse
  • Membrane Proteins
  • Mucoproteins
  • RNA, Messenger
  • Receptors, CXCR5
  • Receptors, Chemokine
  • Receptors, Cytokine
  • integrin alpha4beta7
  • Leukocyte Common Antigens
  • GTP-Binding Proteins