Abstract
Activity of stretch-activated potassium channels has been recorded in articular chondrocytes using patch-clamp technique. Pressure dependence is described by a sigmoidal function with a half-maximum effect at -20.5 mbar. Selectivity for potassium is demonstrated by agreement between the reversal potential measured at different [K+]o and the prediction of Nernst equation and by block of these channels by caesium.
MeSH terms
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Animals
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Cartilage, Articular / physiology*
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Cell Membrane / drug effects
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Cell Membrane / physiology
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Cells, Cultured
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Cesium / pharmacology
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Ion Channel Gating
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Membrane Potentials / drug effects
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Membrane Potentials / physiology
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Patch-Clamp Techniques
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Potassium / pharmacology
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Potassium Channels / drug effects
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Potassium Channels / physiology*
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Stress, Mechanical
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Swine
Substances
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Potassium Channels
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Cesium
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Potassium