PCR-generated cross-over linkers for site-directed mutagenesis

Biotechniques. 1997 Nov;23(5):827-30. doi: 10.2144/97235bm13.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cystic Fibrosis Transmembrane Conductance Regulator / genetics
  • DNA / chemistry
  • DNA / genetics
  • DNA Primers
  • DNA, Complementary / genetics
  • Deoxyribonuclease EcoRI / metabolism
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • Escherichia coli / genetics
  • Exons
  • Humans
  • Introns
  • Mutagenesis, Site-Directed*
  • Plasmids / genetics
  • Polyethylene Glycols
  • Polymerase Chain Reaction*
  • Sequence Analysis, DNA
  • Sequence Homology
  • Transformation, Bacterial

Substances

  • CFTR protein, human
  • DNA Primers
  • DNA, Complementary
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Polyethylene Glycols
  • DNA
  • Deoxyribonuclease EcoRI
  • CTTAAG-specific type II deoxyribonucleases
  • Deoxyribonucleases, Type II Site-Specific