Beraprost sodium, an analogue of prostacyclin, induces the expression of mitogen-activated protein kinase phosphatase and inhibits the proliferation of cultured mesangial cells

Eur J Pharmacol. 1997 Oct 8;336(2-3):291-4. doi: 10.1016/s0014-2999(97)01208-9.

Abstract

Beraprost sodium, an analogue of prostacyclin, increases intracellular cyclic adenosine monophosphate (cAMP) in cultured glomerular mesangial cells. We examined the effect of beraprost on mesangial cell proliferation. Beraprost was able to inhibit fetal bovine serum-stimulated proliferation of mesangial cells in concentrations enough to increase cellular cAMP. By northern blot analysis, beraprost induced the expression of MKP-1, a mitogen-activated protein kinase phosphatase, in a dose- and time-dependent manner, similarly to dibutyryl cAMP and adrenomedullin. These results indicate that beraprost inhibits the proliferation of mesangial cells and one of the mechanisms might be cAMP-dependent induction of MKP-1.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Blotting, Northern
  • Cell Cycle Proteins*
  • Cell Division / drug effects
  • Cells, Cultured
  • Dual Specificity Phosphatase 1
  • Enzyme Induction
  • Epoprostenol / analogs & derivatives*
  • Epoprostenol / pharmacology
  • Glomerular Mesangium / drug effects*
  • Glomerular Mesangium / enzymology
  • Growth Inhibitors / pharmacology*
  • Immediate-Early Proteins / biosynthesis*
  • Male
  • Phosphoprotein Phosphatases*
  • Protein Phosphatase 1
  • Protein Tyrosine Phosphatases / biosynthesis*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Cell Cycle Proteins
  • Growth Inhibitors
  • Immediate-Early Proteins
  • beraprost
  • Epoprostenol
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 1
  • Dual Specificity Phosphatase 1
  • Dusp1 protein, rat
  • Protein Tyrosine Phosphatases