In the present paper, influence of microinjection of clonidine (Clon, 0.38 mMol, 2 microliters) and yohimbine (Yoh, 5.1 mMol, 2 microliters) in rostral ventrolateral medulla (rVLM) on the effect of electroacupuncture (EA) at Neiguan (PC 6) was observed in the rabbit with acute myocardial ischemia. Results showed that (1) EA of Neiguan could significantly accelerate the recovery of AMI-induced changes in ECG ST-segement, mean blood pressure, left ventricular pressure (LVP) and dp/dt max; (2) Clon strengthened the effect of EA on ST and LVP in some degree; and (3) Yoh significantly weakened the effect of EA on ST and LVP. It reveals that when alpha 2 receptors of rVLM are excited, the favourable effect of EA in regulating post-ischemic myocardial electrical and mechanical activity can be enhanced in some degree; and when they are inhibited, the effect of EA disappears, indicating an involvement of alpha 2 receptors of rVLM in EA of Neiguan-induced regulation on the functional activity of the heart.