Involvement of protein kinase C in the activation of extracellular signal-regulated kinase 1/2 by UVC irradiation

Biochem Biophys Res Commun. 1997 Nov 17;240(2):273-8. doi: 10.1006/bbrc.1997.7474.

Abstract

UVC irradiation activates mitogen-activated protein kinases (MAPKs), including ERK, JNK, and P38. This study examined the role of protein kinase C (PKC) in the regulation of UVC-stimulated MAPKs activation. Either the depletion of PKC by prolonged treatment of cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) or the inhibition of PKC by a selective PKC inhibitor, UCN-01-ME, attenuated UVC-activation of ERK1/2, keeping the activation of JNK1/2 intact. However, K252a, a non-selective PKC inhibitor, inhibited the activation of both ERK1/2 and JNK1/2 by UVC. In three isoforms of PKC (alpha, delta, epsilon) examined, PKC epsilon shows the most evident translocation, a temporal association with cell membrane, upon the UVC irradiation of NIH 3T3 cells. These results suggest that PKC is acting in the UVC-dependent activation of ERK1/2, and PKC epsilon is one of the PKC isozymes playing such a role.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Calcium-Calmodulin-Dependent Protein Kinases / radiation effects*
  • Culture Media, Serum-Free
  • Enzyme Activation / radiation effects
  • Humans
  • Isoenzymes / biosynthesis
  • Isoenzymes / metabolism
  • Isoenzymes / radiation effects
  • JNK Mitogen-Activated Protein Kinases
  • Kinetics
  • Mice
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3
  • Mitogen-Activated Protein Kinases*
  • Protein Kinase C / biosynthesis
  • Protein Kinase C / metabolism*
  • Protein Kinase C / radiation effects*
  • Protein Kinase C-alpha
  • Protein Kinase C-delta
  • Protein Kinase C-epsilon
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / radiation effects
  • Transfection
  • Ultraviolet Rays*

Substances

  • Culture Media, Serum-Free
  • Isoenzymes
  • Recombinant Proteins
  • Prkcd protein, mouse
  • Prkce protein, mouse
  • PRKCA protein, human
  • PRKCD protein, human
  • PRKCE protein, human
  • Prkca protein, mouse
  • Protein Kinase C
  • Protein Kinase C-alpha
  • Protein Kinase C-delta
  • Protein Kinase C-epsilon
  • Calcium-Calmodulin-Dependent Protein Kinases
  • JNK Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase 1
  • Mitogen-Activated Protein Kinase 3
  • Mitogen-Activated Protein Kinases