A new generation of serum anti-HIV antibody immunocapture assay for saliva testing

Clin Diagn Virol. 1997 Nov;8(3):195-7. doi: 10.1016/s0928-0197(97)00024-x.

Abstract

Background: Epidemiological studies need easier tools to evaluate HIV prevalence, particularly in high-risk groups under difficult field conditions. Testing saliva antibody with accurate immunoassay could serve as an alternative to serum testing.

Objectives: To evaluate performances on saliva of a novel commercially available assay for anti-HIV antibody.

Study design: Samples of saliva from 530 patients were tested for the presence of HIV antibodies with a third generation commercially available serum-screening kit and the use of a sample diluent adapted to saliva.

Results: Compared with serum sampling the sensitivity and specificity of oral sampling were respectively, 100 and 99.8%.

Conclusion: The ICE HIV-1.0.2 assay used on saliva could be an efficient and non-invasive epidemiological tool for HIV testing.

MeSH terms

  • Adult
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Evaluation Studies as Topic
  • HIV Antibodies / analysis*
  • HIV Infections / diagnosis*
  • Humans
  • Reagent Kits, Diagnostic*
  • Saliva / virology*
  • Sensitivity and Specificity

Substances

  • HIV Antibodies
  • Reagent Kits, Diagnostic