Abstract
A gE-negative bovine herpesvirus 1 (BHV1) vector vaccine carrying a gene coding for the G protein of bovine respiratory syncytial virus (BRSV) (BHV1/BRSV-G) induced the same high degree of protection in calves against BRSV infection and BHV1 infection as a multivalent commercial vaccine. A DNA plasmid vaccine, carrying the same gene as the BHV1/BRSV-G vaccine, significantly reduced BRSV shedding after BRSV infection compared with that in control calves, but less well than the BHV1/BRSV-G vaccine. Flow cytometric analysis showed a significant relative increase of gamma/delta+ T cells in peripheral blood after BRSV challenge-infection of the calves of the control group but not in the vaccinated groups. These results indicate that the G protein of BRSV can induce significant protection against BRSV infection in cattle, and that the BHV1/BRSV-G vaccine protects effectively against a subsequent BRSV and BHV1 infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / immunology
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Cattle
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Cattle Diseases / immunology
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Cattle Diseases / prevention & control*
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Herpesviridae Infections / veterinary*
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Herpesvirus 1, Bovine / genetics
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Herpesvirus 1, Bovine / immunology*
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Respiratory Syncytial Virus Infections / immunology
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Respiratory Syncytial Virus Infections / veterinary*
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Respiratory Syncytial Virus, Bovine / genetics*
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Respiratory Syncytial Virus, Bovine / immunology
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T-Lymphocyte Subsets / immunology
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Vaccination / veterinary*
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Vaccines, Combined / genetics
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Vaccines, Combined / immunology
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Vaccines, Combined / therapeutic use*
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Vaccines, DNA / genetics
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Vaccines, DNA / therapeutic use*
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Viral Envelope Proteins / genetics*
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Viral Envelope Proteins / immunology*
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Viral Vaccines / genetics
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Viral Vaccines / immunology
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Viral Vaccines / therapeutic use*
Substances
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Antibodies, Viral
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G protein, Bovine respiratory syncytial virus
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Vaccines, Combined
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Vaccines, DNA
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Viral Envelope Proteins
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Viral Vaccines