Abstract
Out of a Xenopus neurula cDNA library, we isolated a clone which encodes a 52.4-kDa protein highly similar to the mouse interferon regulatory factor, IRF-6, whose function is unknown. The mRNA of this gene, named xIRF-6, seems to be maternally transmitted, but its amount rapidly decreases after the tailbud stage. Whole-mount in situ hybridization showed that xIRF-6 mRNA is expressed in the presumptive somitic mesoderm in the late gastrula, and then confined to a segment of posterior somite during the neurula through the tailbud stage. The temporally and spatially limited expression of the xIRF-6 gene product may contribute to the transcriptional regulation of specific genes which are necessary for the development of the posterior somites.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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DNA, Complementary
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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Embryo, Nonmammalian / metabolism*
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Gastrula / metabolism
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Gene Expression Regulation, Developmental*
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In Situ Hybridization
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Interferon Regulatory Factors
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Mesoderm / metabolism*
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Molecular Sequence Data
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sequence Analysis, DNA
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Xenopus Proteins*
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Xenopus laevis / embryology
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Xenopus laevis / genetics*
Substances
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DNA, Complementary
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DNA-Binding Proteins
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IRF6 protein, Xenopus
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Interferon Regulatory Factors
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RNA, Messenger
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Transcription Factors
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Xenopus Proteins