Abstract
A full-length cDNA encoding a 113-kDa transcription factor, named P113, was cloned from mouse preadipocyte line 30A5. P113 binds to a 7-bp consensus TNF-response element and a 30-bp fragment from mouse PAI-1 promoter (-88/-59). Sequence analysis indicates that the P113 is highly homologous to HIP116/HLTF (human) and RUSH-1alpha (rabbit). The sequence homology and the fact that P113 contains seven motifs conserved in many DNA-dependent helicases/ATPases indicate that it is a new member of the SNF2/SWI2 protein family. A cysteine-rich motif, called RING finger, was found close to the C-terminus of P113. The expression pattern of P113 mRNA in rat tissues is significantly different from that of HLTF in human tissues. Affinity-purified P113 has an ATPase activity that is activated by DNA in a sequence-specific manner. Using Northern blot analysis and the PAI-1 promoter/luciferase system, we demonstrated that P113 is a transcription factor that activates the transcription of the PAI-1 gene in 30A5 cells.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / metabolism
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Adipocytes
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular
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DNA Helicases*
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / isolation & purification*
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / physiology
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Gene Expression Regulation / drug effects
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HeLa Cells
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Humans
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Mice
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Molecular Sequence Data
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Nuclear Proteins*
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Organ Specificity / genetics
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Plasminogen Activator Inhibitor 1 / genetics
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RNA, Messenger / metabolism
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Rabbits
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Rats
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Sequence Homology, Amino Acid
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Transcription Factors / biosynthesis
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Transcription Factors / genetics*
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Transcription Factors / isolation & purification*
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Transcription Factors / physiology
Substances
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DNA-Binding Proteins
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Nuclear Proteins
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Plasminogen Activator Inhibitor 1
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RNA, Messenger
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SMARCA2 protein, human
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Smarca2 protein, mouse
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Smarca3 protein, mouse
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Transcription Factors
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Adenosine Triphosphatases
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SMARCA4 protein, human
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Smarca4 protein, mouse
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DNA Helicases