Abstract
Mice carrying transgenic rearranged V region genes in their IgH and Igkappa loci to encode an autoreactive specificity direct the emerging autoreactive progenitors into a pre-B cell compartment, in which their receptors are edited by secondary Vkappa-Jkappa rearrangements and RS recombination. Editing is an efficient process, because the mutant mice generate normal numbers of B cells. In a similar nonautoreactive transgenic strain, neither a pre-B cell compartment nor receptor editing was seen. Thus, the pre-B cell compartment may have evolved to edit the receptors of autoreactive cells and later been generally exploited for efficient antibody diversification through the invention of the pre-B cell receptor, mimicking an autoreactive antibody to direct the bulk of the progenitors into that compartment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, CD*
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B-Lymphocytes / immunology*
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Gene Rearrangement, B-Lymphocyte, Heavy Chain / genetics*
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Hematopoietic Stem Cells
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Immune Tolerance*
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin Joining Region / genetics
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Immunoglobulin Light Chains / genetics
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Immunoglobulin M / immunology
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Immunoglobulin Variable Region / genetics
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Immunoglobulin kappa-Chains / genetics
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Immunoglobulin lambda-Chains / genetics
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Leukocyte Common Antigens / immunology
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Leukosialin
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Mice
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Mice, Transgenic
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Mutagenesis, Insertional
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Receptors, Antigen, B-Cell / immunology*
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Sialoglycoproteins / immunology
Substances
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Antigens, CD
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Immunoglobulin Heavy Chains
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Immunoglobulin Joining Region
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Immunoglobulin Light Chains
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Immunoglobulin M
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Immunoglobulin Variable Region
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Immunoglobulin kappa-Chains
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Immunoglobulin lambda-Chains
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Leukosialin
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Receptors, Antigen, B-Cell
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Sialoglycoproteins
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Spn protein, mouse
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Leukocyte Common Antigens