Abstract
Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Base Sequence
-
Cell Line
-
Chromosomes, Bacterial / genetics*
-
DNA Primers / genetics
-
Electroporation*
-
Gene Transfer Techniques
-
Genetic Complementation Test*
-
Genetic Markers
-
Genetic Techniques
-
Humans
-
In Situ Hybridization, Fluorescence
-
Polymerase Chain Reaction
Substances
-
DNA Primers
-
Genetic Markers