Abstract
We examined CD44 isoform expression in 138 frozen tissue samples, which included primary lung carcinomas, adjacent non-tumorous lung tissues and benign lung diseases, by both reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical analyses. CD44v8-10 mRNA and protein were dominantly expressed in non-small cell lung carcinomas (NSCLC), while non-tumorous tissues principally expressed CD44s and small cell lung carcinomas (SCLC) expressed either CD44s or no detectable CD44. These results indicate that CD44v8-10 is the dominant splicing isoform in NSCLC and can be practically utilized as a diagnostic marker and therapeutical target in NSCLC.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aged
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Alternative Splicing*
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Antigens, CD / analysis
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Antigens, CD / biosynthesis*
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Carcinoma, Non-Small-Cell Lung / immunology
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Carcinoma, Non-Small-Cell Lung / pathology*
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Carcinoma, Small Cell / immunology
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Carcinoma, Small Cell / pathology
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Female
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Humans
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Hyaluronan Receptors / analysis
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Hyaluronan Receptors / biosynthesis*
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Immunohistochemistry
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Lung Diseases / immunology
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Lung Diseases / pathology
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Lung Neoplasms / immunology
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Lung Neoplasms / pathology*
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Male
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Middle Aged
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Neoplasm Metastasis
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Polymerase Chain Reaction
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Smoking
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Tumor Cells, Cultured
Substances
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Antigens, CD
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Hyaluronan Receptors