Larvae of Trichinella sp. were found in two of 208 red foxes (Vulpes vulpes) and one of 125 coyotes (Canis latrans) obtained from trappers from Prince Edward Island (Canada) in 1995 and 1996. A polymerase chain reaction based DNA biotyping method revealed the larvae to be isolates of Trichinella spiralis. This is the first verified identification of T. spiralis in sylvatic hosts from Canada.