Internalization of Aeromonas hydrophila by fish epithelial cells can be inhibited with a tyrosine kinase inhibitor

Microbiology (Reading). 1998 Feb:144 ( Pt 2):299-307. doi: 10.1099/00221287-144-2-299.

Abstract

Aeromonas hydrophila is a Gram-negative bacterium that is pathogenic in fish, causing motile aeromonad septicaemia. It can enter (invade) fish cells, and survive as an intracellular parasite. The host-pathogen interaction and signal transduction pathway were studied by screening signal transduction inhibitors using carp epithelial cells and a virulent strain of the bacterium, PPD134/91. Genistein, a tyrosine kinase inhibitor, postponed internalization of A. hydrophila into host cells, suggesting that tyrosine phosphorylation plays a role in internalization. In contrast, staurosporine, a protein kinase C inhibitor, and sodium orthovanadate, a protein tyrosine phosphatase inhibitor, accelerated internalization of PPD134/91. Other virulent strains of A. hydrophila were also examined and it is likely that all strains, irrespective of serogroup, use the same signalling pathway to facilitate bacterial uptake.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Aeromonas hydrophila*
  • Animals
  • Cells, Cultured
  • Enzyme Inhibitors / pharmacology*
  • Epithelial Cells / microbiology
  • Fishes
  • Genistein / pharmacology*
  • Gram-Negative Bacterial Infections / microbiology*
  • Host-Parasite Interactions
  • Microscopy, Confocal
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / metabolism
  • Protein Tyrosine Phosphatases / antagonists & inhibitors
  • Protein Tyrosine Phosphatases / metabolism
  • Protein-Tyrosine Kinases / antagonists & inhibitors*
  • Protein-Tyrosine Kinases / metabolism
  • Signal Transduction
  • Staurosporine / pharmacology
  • Vanadates / pharmacology

Substances

  • Actins
  • Enzyme Inhibitors
  • Vanadates
  • Genistein
  • Protein-Tyrosine Kinases
  • Protein Kinase C
  • Protein Tyrosine Phosphatases
  • Staurosporine