Abstract
PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a convenient technique for the detection of mutations. As the mobility of single-stranded DNA is sequence-dependent it could therefore be used to determine serotype-related sequence variations in Listeria monocytogenes. Sero-specific patterns were observed in different L. monocytogenes serogroups.
MeSH terms
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Bacterial Proteins / genetics*
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DNA Primers
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Genetic Variation
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Humans
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Listeria monocytogenes / classification*
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Listeria monocytogenes / genetics*
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Polymerase Chain Reaction / methods
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Polymorphism, Single-Stranded Conformational*
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Reproducibility of Results
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Serotyping / methods
Substances
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60 kDa protein, Listeria monocytogenes
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Bacterial Proteins
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DNA Primers