Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein

A Crescenzi; L d'Aquino; M Nuzzaci; A Ostuni; A Bavoso; S Comes; A De Stradis; P Piazzolla

doi:10.1016/s0166-0934(97)00158-4

Production of strain specific antibodies against a synthetic polypeptide corresponding to the N-terminal region of the plum pox potyvirus coat protein

J Virol Methods. 1997 Dec;69(1-2):181-9. doi: 10.1016/s0166-0934(97)00158-4.

Authors

A Crescenzi¹, L d'Aquino, M Nuzzaci, A Ostuni, A Bavoso, S Comes, A De Stradis, P Piazzolla

Affiliation

¹ Dipartimento di Biologia, Difesa e Biotecnologie Argo Forestali, Università degli Studi della Basilicata, Potenza, Italy. [email protected]

PMID: 9504763
DOI: 10.1016/s0166-0934(97)00158-4

Abstract

Comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (PPV-SwC) with the corresponding regions of several other PPV strains indicated that the main differences are in the N-terminal region. Polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the N-terminal region of PPV-SwC coat protein. They specifically detected PPV-SwC in different immunochemical tests.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Antibodies, Viral / biosynthesis*
Antibodies, Viral / immunology
Blotting, Western
Capsid / chemistry
Capsid / immunology*
Capsid Proteins*
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Microscopy, Immunoelectron
Molecular Sequence Data
Peptide Fragments / chemical synthesis
Peptide Fragments / chemistry
Peptide Fragments / immunology*
Plant Diseases / virology
Plant Extracts
Potyvirus / immunology*
Potyvirus / isolation & purification*
Sequence Analysis
Species Specificity

Substances

Antibodies, Viral
Capsid Proteins
Peptide Fragments
Plant Extracts
coat protein, Plum pox virus

Associated data

GENBANK/Y09851