Abstract
A cell-free system that mimics the reassembly of Golgi stacks at the end of mitosis requires two ATPases, NSF and p97, to rebuild Golgi cisternae. Morphological studies now show that alpha-SNAP, a component of the NSF pathway, can inhibit the p97 pathway, whereas p47, a component of the p97 pathway, can inhibit the NSF pathway. Anti-syntaxin 5 antibodies and a soluble, recombinant syntaxin 5 inhibited both pathways, suggesting that this t-SNARE is a common component. Biochemical studies confirmed this, showing that p47 binds directly to syntaxin 5 and competes for binding with alpha-SNAP. p47 also mediates the binding of p97 to syntaxin 5 and so plays an analogous role to alpha-SNAP, which mediates the binding of NSF.
MeSH terms
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Adenosine Triphosphatases / metabolism*
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Animals
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Binding, Competitive
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Carrier Proteins / metabolism*
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Cell-Free System
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Cytosol
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Golgi Apparatus / metabolism*
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HeLa Cells
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Humans
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Intracellular Membranes
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Liver
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Membrane Fusion / physiology*
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Membrane Proteins / metabolism*
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Mitosis
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N-Ethylmaleimide-Sensitive Proteins
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Protein Binding
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Qa-SNARE Proteins
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Rats
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Recombinant Fusion Proteins / metabolism
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Vesicular Transport Proteins*
Substances
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Carrier Proteins
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Membrane Proteins
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Nsfl1c protein, rat
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Qa-SNARE Proteins
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Recombinant Fusion Proteins
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Vesicular Transport Proteins
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Adenosine Triphosphatases
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N-Ethylmaleimide-Sensitive Proteins
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Nsf protein, rat