Thrombopoietin stimulates VEGF release from c-Mpl-expressing cell lines and haematopoietic progenitors

FEBS Lett. 1998 Feb 13;423(1):10-4. doi: 10.1016/s0014-5793(98)00056-8.

Abstract

Vascular endothelial growth factor (VEGF) production was analysed in megakaryocytic cell lines and CD34+ haematopoietic progenitors following treatment with thrombopoietin (TPO). In CMK cells TPO caused a time- and dose-dependent increase in the levels of VEGF released into the medium. A similar effect was observed in UT-7/mpl cells transfected with the TPO receptor c-Mpl, but not in parental UT-7 cells. In CD34+ haematopoietic progenitor cell cultures TPO stimulated VEGF mRNA expression and VEGF protein release. Production of VEGF in CD34+ cultures increased with TPO-induced megakaryocytic differentiation, but not with erythroid or myelomonocytic differentiation induced respectively by erythropoietin and granulocyte-macrophage colony-stimulating factor. These results demonstrate that TPO stimulates VEGF release in c-Mpl-expressing cells and suggest that this process is an integral feature of the megakaryocytic differentiation programme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Line
  • Cells, Cultured
  • Culture Media
  • Endothelial Growth Factors / biosynthesis
  • Endothelial Growth Factors / genetics
  • Endothelial Growth Factors / metabolism*
  • Gene Expression
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / drug effects*
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Lymphokines / biosynthesis
  • Lymphokines / genetics
  • Lymphokines / metabolism*
  • Megakaryocytes / cytology
  • Megakaryocytes / drug effects*
  • Megakaryocytes / metabolism
  • Neoplasm Proteins*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • RNA, Messenger
  • Receptors, Cytokine*
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / metabolism*
  • Receptors, Thrombopoietin
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / pharmacology
  • Thrombopoietin / metabolism*
  • Thrombopoietin / pharmacology
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors

Substances

  • Culture Media
  • Endothelial Growth Factors
  • Lymphokines
  • Neoplasm Proteins
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Receptors, Cytokine
  • Receptors, Immunologic
  • Receptors, Thrombopoietin
  • Recombinant Proteins
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • MPL protein, human
  • Thrombopoietin