Inhibition of forskolin-induced sensitisation of frog skin nociceptors by the cyclic AMP-dependent protein kinase A antagonist H-89

Brain Res. 1998 Jan 12;780(2):360-2. doi: 10.1016/s0006-8993(97)01360-7.

Abstract

The role of cAMP and protein kinase A (PKA) in nociceptor sensitisation has been studied in frog skin in vitro. Multifibre nerve responses during noxious heating were enhanced by adding forskolin, an agent that elevates cAMP. H-89, a PKA antagonist, blocked the increased responses due to forskolin, but did not affect baseline responses. Thus, cAMP appears to act via PKA when sensitising nociceptors in frog skin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / drug effects
  • Action Potentials / physiology
  • Animals
  • Colforsin / pharmacology*
  • Cyclic AMP / metabolism
  • Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors*
  • Enzyme Inhibitors / pharmacology*
  • Hot Temperature
  • Hyperalgesia / metabolism
  • Isoquinolines / pharmacology*
  • Neurons, Afferent / drug effects
  • Neurons, Afferent / enzymology
  • Nociceptors / drug effects*
  • Rana pipiens
  • Skin / innervation
  • Sulfonamides*

Substances

  • Enzyme Inhibitors
  • Isoquinolines
  • Sulfonamides
  • Colforsin
  • Cyclic AMP
  • Cyclic AMP-Dependent Protein Kinases
  • N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide