Intra-assay performance characteristics of five assays for quantification of human immunodeficiency virus type 1 RNA in plasma

J Clin Microbiol. 1998 Mar;36(3):835-9. doi: 10.1128/JCM.36.3.835-839.1998.

Abstract

Three kits (Roche AMPLICOR human immunodeficiency virus type 1 [HIV-1] Monitor, Chiron enhanced-sensitivity bDNA, and Organon Teknika NASBA HIV-1 QT) and two in-house assays (from National Genetics Institute and Baylor College of Medicine) were compared with a blinded panel. The results were evaluated as to intra-assay sensitivity, precision, and ability to detect differences in a dilution series.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Evaluation Studies as Topic
  • False Positive Reactions
  • HIV Infections / virology*
  • HIV-1 / genetics
  • HIV-1 / physiology*
  • Humans
  • RNA, Viral / blood*
  • Reagent Kits, Diagnostic
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Viral Load*
  • Viremia

Substances

  • RNA, Viral
  • Reagent Kits, Diagnostic