Abstract
Somatic hypermutation and subsequent selection play a significant role in shaping the peripheral B cell repertoire. This repertoire is composed of CD5+ (5%) and CD5- B cells (95%) which are known to traffic through different lymphoid compartments. Previous studies have shown that V(H) gene usage by CD5+ and CD5- B cells is similar, although mutations are more frequent in the latter. However, the effect of mutation and subsequent selection on the expressed V(H) repertoire of CD5+ and CD5- B cells has not been delineated in detail. This study, therefore, analyzed the mutational pattern of individual IgM+/CD5+ and IgM+/CD5- B cells. In both populations, mutations can occur without heavy chain isotype switching. Despite the differences in mutational frequency, the patterns of mutation and subsequent selection were comparable in CD5+ and CD5- B cells. These results imply that although mutations are more frequent in CD5- B cells, the overall mechanisms governing somatic hypermutation and subsequent positive and negative selection are similar in CD5+ and CD5- B cells.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adenine
-
Adult
-
B-Lymphocyte Subsets / immunology*
-
B-Lymphocyte Subsets / metabolism*
-
CD5 Antigens / genetics*
-
Codon / genetics
-
Cytosine
-
DNA Mutational Analysis / statistics & numerical data
-
Gene Rearrangement, B-Lymphocyte, Heavy Chain / genetics
-
Guanine
-
Humans
-
Immunoglobulin Heavy Chains / biosynthesis
-
Immunoglobulin Heavy Chains / genetics
-
Immunoglobulin Joining Region / biosynthesis
-
Immunoglobulin Joining Region / genetics
-
Immunoglobulin M / genetics*
-
Immunoglobulin Variable Region / biosynthesis
-
Immunoglobulin Variable Region / genetics
-
Male
-
Middle Aged
-
Molecular Sequence Data
-
Mutation*
-
Purine Nucleotides / genetics
-
Serine / genetics
-
Statistics, Nonparametric
Substances
-
CD5 Antigens
-
Codon
-
Immunoglobulin Heavy Chains
-
Immunoglobulin Joining Region
-
Immunoglobulin M
-
Immunoglobulin Variable Region
-
Purine Nucleotides
-
Serine
-
Guanine
-
Cytosine
-
Adenine
Associated data
-
GENBANK/Z80363
-
GENBANK/Z80364
-
GENBANK/Z80365
-
GENBANK/Z80366
-
GENBANK/Z80367
-
GENBANK/Z80368
-
GENBANK/Z80369
-
GENBANK/Z80370
-
GENBANK/Z80371
-
GENBANK/Z80372
-
GENBANK/Z80373
-
GENBANK/Z80374
-
GENBANK/Z80375
-
GENBANK/Z80376
-
GENBANK/Z80377
-
GENBANK/Z80378
-
GENBANK/Z80379
-
GENBANK/Z80380
-
GENBANK/Z80381
-
GENBANK/Z80382
-
GENBANK/Z80383
-
GENBANK/Z80384
-
GENBANK/Z80385
-
GENBANK/Z80386
-
GENBANK/Z80387
-
GENBANK/Z80388
-
GENBANK/Z80389
-
GENBANK/Z80390
-
GENBANK/Z80391
-
GENBANK/Z80392