Physiological effects of a pea protein isolate in gnotobiotic rats: comparison with a soybean isolate and meat

Ann Nutr Metab. 1998;42(1):44-54. doi: 10.1159/000012717.

Abstract

Pea proteins have been considered for the introduction into the human diet only recently. This protein source was tested on nutritional and digestive parameters in heteroxenic male Fischer rats inoculated with a human faecal microflora from a methane producer. Compared to soybean proteins, pea proteins have similar effects on the rat's endogenous and bacterial digestive patterns. Compared to the pea proteins, a diet containing a standard meat meal enhanced the pH and the production of ammonia, while a lyophilized beef meat enhanced that of urea. The diet containing the standard meat decreases short-chain fatty acids and modifies the ratio of caecal short-chain fatty acids. Both animal diets decreased the specific activities of pancreatic proteases such as chymotrypsin (EC 3.4.21.1), trypsin (EC 3.4.21.4), and carboxypeptidase A (EC 3.4.17.1) when compared to the diet containing the pea isolate. In conclusion, the whole composition of the diet, more than the origin of the dietary protein, influences the rat's digestive pattern.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Nutritional Physiological Phenomena*
  • Animals
  • Cecum / metabolism
  • Cecum / microbiology
  • Cohort Studies
  • Diet
  • Dietary Proteins / administration & dosage
  • Dietary Proteins / metabolism*
  • Feces / microbiology
  • Germ-Free Life / physiology*
  • Glycine max*
  • Humans
  • Intestinal Mucosa / enzymology
  • Intestinal Mucosa / growth & development
  • Male
  • Meat*
  • Methanobacterium / metabolism
  • Organ Size
  • Pancreas / chemistry
  • Pancreas / enzymology
  • Pancreas / growth & development
  • Pisum sativum*
  • Plant Proteins / administration & dosage
  • Plant Proteins / metabolism*
  • Proteins / analysis
  • Rats
  • Rats, Inbred F344

Substances

  • Dietary Proteins
  • Plant Proteins
  • Proteins