Abstract
The full-length mouse RNA polymerase II (pol II) largest subunit (RPB1) gene was used to replace 5070 bp of the yeast Saccharomyces cerevisiae RPB1 gene via homologous recombination and gene replacement in vivo. Transcription of the mouse RPB1 gene using the yeast promoter in the haploid state was confirmed by Northern analysis. This strain of yeast is viable, indicating that mouse RPB1 is able to interact functionally with the other yeast RNA pol II subunits in vivo.
MeSH terms
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Animals
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Base Sequence
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Cloning, Molecular
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DNA Primers
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Escherichia coli
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Genotype
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Macromolecular Substances
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Mice
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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Protein Biosynthesis
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RNA Polymerase II / biosynthesis*
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RNA Polymerase II / chemistry*
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RNA Polymerase II / genetics
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Recombination, Genetic
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Restriction Mapping
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics*
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Transcription, Genetic*
Substances
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DNA Primers
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Macromolecular Substances
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Recombinant Fusion Proteins
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RNA Polymerase II