Abstract
Two SHIVs with two or three genes deleted (SHIV-drn and SHIV-dxrn) were constructed. The inoculation of monkeys with SHIV-drn resulted in short-term viremia, but inoculation with SHIV-dxrn did not. At 68 weeks post-inoculation, the monkeys were reinoculated with a 100-fold higher dose of each SHIV, but none showed viremia. Killer cell activities against HIV-1 Env were detected in the SHIV-drn- and SHIV-dxrn-inoculated monkeys. Cross-reactive killer activity against HIV-1 Gag and SIVmac Gag was observed in one monkey. Antibodies were not detected in the SHIV-dxrn-inoculated monkeys, but the SHIV-drn-inoculated monkeys showed an anamnestic antibody reaction. These data indicate that SHIV-drn is infectious to and immuno-inducible in macaques but SHIV-dxrn is not.
MeSH terms
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AIDS Vaccines / immunology
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Acquired Immunodeficiency Syndrome / prevention & control
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Animals
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Antibodies, Viral / biosynthesis
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Cross Reactions
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Cytotoxicity, Immunologic
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Gene Deletion
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Gene Products, env / genetics
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Gene Products, env / immunology
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Gene Products, gag / genetics
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Gene Products, gag / immunology
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Genes, Viral
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Genes, nef
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Genes, vpr
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HIV Antibodies / biosynthesis
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HIV-1 / genetics*
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HIV-1 / immunology*
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Immunologic Memory
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Macaca
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Reassortant Viruses / genetics*
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Reassortant Viruses / immunology*
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Recombinant Fusion Proteins
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Simian Acquired Immunodeficiency Syndrome / prevention & control
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Simian Acquired Immunodeficiency Syndrome / virology
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Simian Immunodeficiency Virus / genetics*
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Simian Immunodeficiency Virus / immunology*
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Simian Immunodeficiency Virus / physiology
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Vaccines, Attenuated / immunology
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Viral Load
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Viral Regulatory and Accessory Proteins / genetics
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Viremia
Substances
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AIDS Vaccines
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Antibodies, Viral
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Gene Products, env
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Gene Products, gag
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HIV Antibodies
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Recombinant Fusion Proteins
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VPX protein, Human immunodeficiency virus 2
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VPX protein, Simian immunodeficiency virus
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Vaccines, Attenuated
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Viral Regulatory and Accessory Proteins