Doppler tissue imaging (DTI) is an adaptation of the color-doppler, which allows the measurement of low-speed myocardial wall movement. We describe the case of a 51-year-old woman suffering from cardiac amyloidosis with serious endangerment of the diastolic function and mitral flow velocity pattern that was indistinguishable from the normal. The protodiastolic speed of the myocardial walls was measured with pulsed DTI, which was used as a diastolic function index. In this patient, the speed was 5 cm/sec, which was markedly lower than the values found in normal subjects and published recently. Moreover, the DTI M-mode images are examined here in order to point out different characteristics compared to the ones that can be obtained in normal subjects. This therefore exemplifies the possible use of this new technique in studying diastolic function.