A Xenopus cysteine string protein with a cysteine residue in the J domain

Biochim Biophys Acta. 1998 Mar 5;1401(3):239-41. doi: 10.1016/s0167-4889(97)00160-2.

Abstract

A cDNA clone encoding a Xenopus cysteine string protein (Xcsp) was isolated and sequenced. The deduced primary sequence of Xcsp is very similar to other vertebrate csps with the exception of a cysteine residue that lies outside of the cysteine-string domain. This cysteine residue replaces a serine that is highly conserved among vertebrate csps, and thus may be of functional importance. Xcsp mRNA appears as a 4.6 kb species on Northern analysis, and immunoblot of Xenopus brain membranes reveals a single, 35 kDa Xcsp that can be deacylated, like other csps.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain Chemistry
  • Cloning, Molecular
  • Cysteine / genetics*
  • DNA, Complementary / genetics
  • HSP40 Heat-Shock Proteins
  • Membrane Proteins*
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics*
  • RNA, Messenger / analysis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Xenopus laevis / genetics*

Substances

  • DNA, Complementary
  • HSP40 Heat-Shock Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • cysteine string protein
  • Cysteine

Associated data

  • GENBANK/AF015662