Abstract
We report the identification of the proteins encoded by the mttABC operon (formerly yigTUW), which mediate a novel Sec-independent membrane targeting and translocation system in Escherichia coli that interacts with cofactor-containing redox proteins having a S/TRRXFLK "twin arginine" leader motif. A pleiotropic-negative mutant in mttA prevents the periplasmic localization of twin arginine redox enzymes, including nitrate reductase (NapA) and trimethylamine N-oxide reductase (TorA). The mutation also prevents the correct localization of the integral membrane molybdoenzyme dimethylsulfoxide reductase (DmsABC). The DmsA subunit has a twin arginine leader. Proteins with a Sec-dependent leader or which assemble spontaneously in the membrane are not affected by this mutation. MttA, B, and C are members of a large family of related sequences extending from archaebacteria to higher eukaryotes.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
2S Albumins, Plant
-
Amino Acid Sequence
-
Arginine / metabolism
-
Cell Membrane / enzymology
-
Cloning, Molecular
-
Escherichia coli / genetics
-
Escherichia coli / growth & development
-
Escherichia coli / metabolism*
-
Escherichia coli Proteins
-
Genotype
-
Iron-Sulfur Proteins*
-
Kinetics
-
Membrane Proteins / biosynthesis*
-
Membrane Proteins / chemistry
-
Membrane Proteins / genetics
-
Membrane Transport Proteins*
-
Molecular Sequence Data
-
NADH, NADPH Oxidoreductases / metabolism*
-
Nitrate Reductase
-
Nitrate Reductases / metabolism*
-
Operon*
-
Oxidoreductases / metabolism*
-
Oxidoreductases Acting on CH-NH Group Donors
-
Plant Proteins / metabolism*
-
Plasmids
-
Polymerase Chain Reaction
-
Protein Sorting Signals / chemistry
-
Protein Structure, Secondary
-
Recombinant Proteins / biosynthesis
-
Recombinant Proteins / chemistry
-
Sequence Alignment
Substances
-
2S Albumins, Plant
-
Escherichia coli Proteins
-
Iron-Sulfur Proteins
-
Membrane Proteins
-
Membrane Transport Proteins
-
Plant Proteins
-
Protein Sorting Signals
-
Recombinant Proteins
-
napin protein, Brassica napus
-
Arginine
-
Oxidoreductases
-
methylamine dehydrogenase
-
Oxidoreductases Acting on CH-NH Group Donors
-
NADH, NADPH Oxidoreductases
-
Nitrate Reductases
-
Nitrate Reductase
-
napA protein, E coli
-
dimethyl sulfoxide reductase