Abstract
A better understanding of the causative agent's biology and the definition of new targets for the development of drugs and/or specific immune responses is necessary to face the spred of drug-resistant malaria in developing countries and the absence of an efficient vaccine against this most important infectious disease. Non-detergent sulphobetaines enhance the recovery and isoelectric focussing of active Plasmodium falciparum proteases, cytoskeleton-associated proteins and Maurer's cleft-associated proteins. This is a significant advantage for the purification of such proteins and might help pinpoint their role for red blood cell rupture and merozoite release.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Betaine / analogs & derivatives*
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Blood Proteins / isolation & purification*
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Cytoskeletal Proteins / blood
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Cytoskeletal Proteins / isolation & purification*
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Cytoskeleton / parasitology
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Cytoskeleton / physiology
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Endopeptidases / blood
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Endopeptidases / isolation & purification*
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Erythrocyte Membrane / parasitology
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Erythrocyte Membrane / physiology
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Erythrocytes / parasitology*
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Erythrocytes / physiology*
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Indicators and Reagents
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Isoelectric Focusing
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Membrane Proteins / blood
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Membrane Proteins / isolation & purification*
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Molecular Weight
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Plasmodium falciparum / pathogenicity
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Plasmodium falciparum / physiology*
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Protozoan Proteins / blood
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Protozoan Proteins / isolation & purification*
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Solubility
Substances
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Blood Proteins
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Cytoskeletal Proteins
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Indicators and Reagents
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Membrane Proteins
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Protozoan Proteins
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Betaine
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Endopeptidases