Abstract
Incorporation of Vpx into human immunodeficiency virus type 2 (HIV-2) virus-like particles is mediated by the Gag polyprotein. We have identified residues 15 to 40 of Gag p6 and residues 73 to 89 of Vpx as being necessary for virion incorporation. In addition, we show enhanced in vitro binding of Vpx to a chimeric HIV-1/HIV-2 Gag construct containing residues 2 to 49 of HIV-2 p6 and demonstrate that the presence of residues 73 to 89 of Vpx allows for in vitro binding to HIV-2 Gag.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Binding Sites
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HIV Core Protein p24 / genetics
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HIV Core Protein p24 / metabolism*
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HIV-2 / metabolism*
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Humans
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Molecular Sequence Data
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Retroviridae Proteins / genetics
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Retroviridae Proteins / metabolism
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Viral Regulatory and Accessory Proteins / genetics
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Viral Regulatory and Accessory Proteins / metabolism*
Substances
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HIV Core Protein p24
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Retroviridae Proteins
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VPX protein, Human immunodeficiency virus 2
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Viral Regulatory and Accessory Proteins