In order to study the cell origin of acute leukemia with lack of lineage specific antigen expression, the dynamic change of cell surface markers in 25 patients of acute leukemia unclassified with immunologic criteria were analysed by using APAAP immunoenzymatic method with monoclonal antibodies after chemotherapy or relapse. The results showed that 5 patients, whose leukemic cells expressed only CD38 antigen in the first visit, expressed T cell differentiated antigen after chemotherapy or relapse. 4 patients, who expressed B cell specific markers after chemotherapy or relapse, all expressed only CD9 antigen in the first visit. 4 of the 6 patients who expressed only HLA-DR antigen were found to have B cell markers on their leukemic cells surface after chemotherapy or relapse. One patient expressed B cell surface markers after chemotherapy and another expressed T cell surface markers after relapse, both of them expressed CD38 and HLA-DR antigens in the first visit. In 4 other patients whose leukemic cells were lacking of in any cell differentiated antigen expressing, one expressed B cell markers and another expressed T cell markers after chemotherapy. The change of cell surface markers showed the evolution and progression of leukemic cell clone. Dynamic study is useful to distinguish the cell origin of leukemia.