Abstract
Lunatic Fringe (l-Fng) is one of the vertebrate homologues of Drosophila Fringe, which interacts with the Notch signal pathway and regulates activation of the Notch ligands, Delta and Serrate. To elucidate the roles of l-Fng in vertebrate neurogenesis, we transfected chick l-Fng (C-l-Fng) to chick neural tube using the in ovo electroporation technique and examined the subsequent changes in expression of Notch-related genes. We observed downregulation of C-Serrate-1 by ectopic C-l-Fng expression which implied that C-l-Fng acts on the vertebrate Notch pathway to regulate the expression of its ligand.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Avian Proteins
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Calcium-Binding Proteins
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Chick Embryo
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Down-Regulation / genetics*
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Drosophila Proteins
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Drosophila melanogaster
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Electrodes
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Electroporation* / methods
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Gene Expression Regulation, Developmental
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Glycosyltransferases*
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Intercellular Signaling Peptides and Proteins
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Jagged-1 Protein
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Membrane Proteins / biosynthesis*
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Membrane Proteins / genetics
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Nervous System / embryology*
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Nervous System / metabolism*
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Protein Biosynthesis*
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Proteins / genetics*
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Proteins / physiology
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Serrate-Jagged Proteins
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Signal Transduction / genetics
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Transfection* / methods
Substances
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Avian Proteins
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Calcium-Binding Proteins
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Drosophila Proteins
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Intercellular Signaling Peptides and Proteins
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Jagged-1 Protein
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Membrane Proteins
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Proteins
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Ser protein, Drosophila
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Serrate-Jagged Proteins
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Glycosyltransferases
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LFNG protein, Gallus gallus