Abstract
The HIV-1 subtype distribution in 83 HIV-1-seropositive individuals living in Greece was investigated by using the heteroduplex mobility assay (HMA), DNA sequencing, and phylogenetic analysis. The results revealed that partial HIV-1 gp120 sequences from 71 (86%) patients were subtype B, 5 (6%) were subtype A, 4 were subtype D (5%), 2 (2%) were subtype C, and 1 (1%) was subtype I. The subtype I isolate was documented in an intravenous drug user. A high prevalence (90-100%) of B isolates among intravenous drug users, hemophiliacs, and homosexual men was observed, in contrast to heterosexuals, among whom non-B subtypes seemed to be common (42.9%, p < 0.001). Among the Greek population subtype B is the most frequent (94%), in contrast to the high prevalence (57%) of non-B isolates found in emigrants living in Greece (p < 0.001). A heterosexual transmission case of subtype D in a Greek individual not traveling abroad was also documented. The broad HIV-1 diversity in Greece may be explained by population movements, such as migration and traveling.
Publication types
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Multicenter Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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DNA, Viral / blood
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Emigration and Immigration
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Female
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Genotype
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Greece / epidemiology
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HIV Envelope Protein gp120 / genetics
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HIV Seropositivity / epidemiology
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HIV Seropositivity / transmission
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HIV Seropositivity / virology*
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HIV-1 / genetics*
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Homosexuality, Male
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Humans
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Male
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Middle Aged
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Molecular Epidemiology
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Nucleic Acid Heteroduplexes
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Phylogeny
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Polymerase Chain Reaction / methods
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Prevalence
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Sequence Analysis, DNA
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Substance Abuse, Intravenous
Substances
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DNA, Viral
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HIV Envelope Protein gp120
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Nucleic Acid Heteroduplexes
Associated data
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GENBANK/AF049290
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GENBANK/AF049291
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GENBANK/AF049292
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GENBANK/AF049293
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GENBANK/AF049294
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GENBANK/AF049295
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GENBANK/AF049296
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GENBANK/AF049297
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GENBANK/AF049298
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GENBANK/AF049299
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GENBANK/AF049300
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GENBANK/AF049301
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GENBANK/AF049302
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GENBANK/AF049303
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GENBANK/AF049304
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GENBANK/AF049305
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GENBANK/AF049306
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GENBANK/AF049307
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GENBANK/AF049308
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GENBANK/AF049309
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GENBANK/AF049310
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GENBANK/AF049311
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GENBANK/AF049312
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GENBANK/AF049313
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GENBANK/AF049314
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GENBANK/AF049315
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GENBANK/AF049316
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GENBANK/AF049317
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GENBANK/AF049318
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GENBANK/AF049319