Abstract
We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, Bacterial / genetics*
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Antigens, Surface / genetics
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Chlorocebus aethiops
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DNA, Complementary
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Gene Expression*
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Insect Vectors / microbiology
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Polymerase Chain Reaction / methods*
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RNA, Messenger / genetics
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Rickettsia typhi / genetics*
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Rickettsia typhi / growth & development
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Siphonaptera / microbiology
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Vero Cells
Substances
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Antigens, Bacterial
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Antigens, Surface
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DNA, Complementary
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RNA, Messenger