Protective immunization of monkeys with BCG or BCG plus heat-killed Mycobacterium leprae: clinical results

Lepr Rev. 1998 Mar;69(1):6-23. doi: 10.5935/0305-7518.19980003.

Abstract

Rhesus and sooty mangabey monkeys (RM and SMM) were vaccinated and boosted with BCG or BCG + low dose (LD) or high dose (HD) heat-killed Mycobacterium leprae (HKML). One group was not vaccinated. Except for a group of controls, all monkeys were challenged with live M. leprae. All animals were studied longitudinally to determine antileprosy protective efficacy. BCG reduced the numbers of RM with histopathologically-diagnosed leprosy by 70% and slowed and ameliorated the appearance of symptoms. BCG + LDHKML reduced the number of RM with leprosy by 89% and BCG + HDHKML by 78%. BCG did not protect SMM from developing leprosy, but disease progress was slowed; disease in SMM was exacerbated by the addition of HKML to the vaccine. RM, as a species, are prone to paucibacillary (PB) forms of leprosy, whereas SMM are prone to multibacillary (MB) forms. Thus, BCG vaccination offers significant protection from clinical disease and slows/ameliorates the rate of progression/degree of disease at the PB end and appears to at least ameliorate symptoms at the MB end of the leprosy spectrum. BCG + HKML protects at the PB end and exacerbates disease progress at the MB end of the leprosy spectrum.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Bacterial / blood
  • BCG Vaccine / administration & dosage*
  • Bacterial Vaccines / administration & dosage*
  • Disease Models, Animal
  • Female
  • Glycolipids / blood
  • Haplorhini
  • Immunization / methods*
  • Leprosy / immunology
  • Leprosy / prevention & control*
  • Longitudinal Studies
  • Macaca mulatta
  • Male
  • Mycobacterium leprae / immunology*
  • Reference Values
  • Software
  • Vaccines, Inactivated / administration & dosage

Substances

  • Antigens, Bacterial
  • BCG Vaccine
  • Bacterial Vaccines
  • Glycolipids
  • Vaccines, Inactivated
  • phenolic glycolipid I, Mycobacterium leprae