Abstract
The Kluyveromyces lactis HIS4 gene was cloned by complementation of a Saccharomyces cerevisiae his4 mutant. Sequence analysis revealed a 2388 bp open reading frame encoding a single polypeptide predicted to encompass three distinct enzymatic activities (phosphoribosyl-AMP cyclohydrolase, phosphoribosyl-ATP pyrophosphohydrolase and histidinol dehydrogenase). This structural organization is strikingly similar to that of the His4 proteins from S. cerevisiae and Pichia pastoris. Transcript analysis detected a single mRNA species of 2.5 kb.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alcohol Oxidoreductases
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Amino Acid Sequence
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Aminohydrolases
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Cloning, Molecular
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Fungal Proteins / chemistry
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Fungal Proteins / genetics*
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Kluyveromyces / chemistry
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Kluyveromyces / genetics*
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Molecular Sequence Data
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Pyrophosphatases
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RNA, Messenger / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Analysis, DNA
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Structure-Activity Relationship
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Transcription Factors / chemistry
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Transcription Factors / genetics*
Substances
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Fungal Proteins
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RNA, Messenger
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Alcohol Oxidoreductases
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HIS4 protein, S cerevisiae
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Aminohydrolases
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Pyrophosphatases