Abstract
Inhibition of most of the expression of the cysteine proteinases of Entamoeba histolytica strain HM-1:IMSS was successfully performed by transcription of ehcp5 antisense RNA using the promoter of ehg34, which encodes a L21 ribosomal protein of E. histolytica. We have generated a stable transfectant in which the overall level of cysteine proteinase activity is strongly reduced ( 90%). This transfectant has a normal growth rate in Diamond's TYI-S-33 medium, a cytopathic and haemolytic activity similar to the control HM-1:IMSS pEhAct-Neo transfectant but with a significantly lower phagocytic activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acrylic Resins
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Alcohol Dehydrogenase / metabolism
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Animals
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Culture Media
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Cysteine Endopeptidases / genetics
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Cysteine Endopeptidases / metabolism*
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Entamoeba histolytica / enzymology*
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Entamoeba histolytica / genetics
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Entamoeba histolytica / physiology*
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Erythrocytes / metabolism
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Gelatinases / metabolism
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Gene Expression Regulation, Enzymologic / drug effects*
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Genes, Protozoan
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Helminth Proteins*
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Hemolytic Plaque Technique
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Humans
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Phagocytosis
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RNA, Antisense / pharmacology*
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Transcription, Genetic
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Transfection
Substances
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Acrylic Resins
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Culture Media
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Helminth Proteins
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RNA, Antisense
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polyacrylamide gels
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Alcohol Dehydrogenase
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Cysteine Endopeptidases
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Gelatinases