31P NMR study of the interactions between oligodeoxynucleotides containing (6-4) photoproduct and Fab fragments of monoclonal antibodies specific for (6-4) photoproduct

FEBS Lett. 1998 Jun 12;429(2):157-61. doi: 10.1016/s0014-5793(98)00582-1.

Abstract

A 31P nuclear magnetic resonance (NMR) study of the interactions between oligonucleotides containing the (6-4) photoproduct and the Fab fragments of monoclonal antibodies (64M3 and 64M5) recognizing the (6-4) photoproduct is reported. The 31P chemical shift data indicate that backbone conformation of (64) adduct is affected by the presence of flanking oligodeoxynucleotides, and (6-4) adducts with different backbone conformations are accommodated in the antigen binding sites of these antibodies. It was also revealed that epitopes for these antibodies consist of not only the (6-4) adduct but the flanking di- or tri-deoxynucleotides on both the 5' and 3' sides as well.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • Antigen-Antibody Reactions
  • DNA Adducts
  • Epitope Mapping*
  • Immunoglobulin Fab Fragments / immunology*
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Nucleic Acid Conformation
  • Phosphorus Isotopes
  • Pyrimidine Dimers / chemistry
  • Pyrimidine Dimers / immunology*

Substances

  • Antibodies, Monoclonal
  • DNA Adducts
  • Immunoglobulin Fab Fragments
  • Phosphorus Isotopes
  • Pyrimidine Dimers
  • pyrimidine-pyrimidone dimer